◦c anti ph3 antibody  (Cell Signaling Technology Inc)

Journal: Cell Death Discovery

Article Title: GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation

doi: 10.1038/s41420-026-03059-1

Figure Lengend Snippet: a Schematic of the experimental procedure used. Spint1a-deficient embryos were treated with Palbociclib or vehicle (DMSO) at 1 dpf by bath immersion. At 2 dpf, images were taken to analyze Nfkb reporter expression and neutrophil distribution. Anti-pH3 immunofluorescence was also performed. b Representative images of 2 dpf control and treated larvae, showing neutrophils and Nfkb reporter activity. c Quantification of Nfkb activity in skin in each group. Quantification of distribution ( d ) and number ( e ) of neutrophils in tail in every condition. e Quantification of neutrophil distribution in the tail in every condition. f Representative images of anti-pH3 immunofluorescence of 2 dpf control and treated larvae. g Number of pH3 + cells in skin in every condition. h Analysis of the correlation between the number of neutrophils and the number of pH3 + cells in skin. Each dot represents one individual, and the means and SEM for each group are also shown. P values were calculated by Student’s t test ( c , d , f ) and by Pearson Correlation test ( g ). ns, not significant; * P ≤ 0.05 ; *** P ≤ 0.001.

Article Snippet: Following, they were incubated in anti-Phospho-Histone H3 (pH3) antibody (Cell Signaling, #9701, 1:100) diluted in blocking solution for two days at 4 °C.

Techniques: Expressing, Immunofluorescence, Control, Activity Assay